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Silica gel is a form of silicon dioxide, which is widely used in different products. It is also known as a drying agent. It has wide surface area, which helps in removing moisture from the solid. In addition, silica gel is odorless.
Several commercial applications of silica gel exist, including drying agent and chromatography. Its chemical stability and adsorbing ability have made it suitable for many uses. A wide variety of products, such as jerky, potato storage drawers, and garlic drawers, are benefiting from its use.
The adsorption capacity of silica particles depends on the buffer composition. The positively charged amino acid buffers show promise as a potential alternative to chaotropic salts for adsorption in the solid phase. However, the amount of DNA adsorbed to the particle is limited by the non-polar acidity of the buffer.
DNA adsorption to silica was investigated in an experiment using a mixed pH and polarity amino acid buffer. The adsorption capacity was lower for silica particles with negative charges, while the overall elution yield was higher for the positively charged amino acid buffers.
The QCM-D characterization of the DNA elution from the silica surface indicates that the initial elution is partially dehydrated. This is likely due to residual solution left behind during the adsorption process.
The adsorption of DNA to silica particles may be driven by interaction between the amino acid buffer and the silica surface. During the early stages of adsorption, the DNA film is more rigid. But it is relatively flexible during the later stages of adsorption.
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